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| Link zu diesem Datensatz | https://d-nb.info/1343963656 |
| Titel | Golden Gate Cloning : Methods and Protocols / edited by Daniel Schindler |
| Person(en) | Schindler, Daniel (Herausgeber) |
| Organisation(en) | SpringerLink (Online service) (Sonstige) |
| Ausgabe | 1st ed. 2025 |
| Verlag | New York, NY : Springer US, Imprint: Humana |
| Zeitliche Einordnung | Erscheinungsdatum: 2025 |
| Umfang/Format | Online-Ressource, X, 470 p. 93 illus., 90 illus. in color. : online resource. |
| Andere Ausgabe(n) |
Printed edition:: ISBN: 978-1-0716-4094-4 Printed edition:: ISBN: 978-1-0716-4221-4 Printed edition:: ISBN: 978-1-0716-4222-1 |
| Inhalt | Golden Gate Cloning of MoClo Standard Parts -- Assembly of Multigene Constructs using the Modular Cloning System MoClo -- Selection of Fusion-Site Overhang Sets for High Fidelity and High Complexity Golden Gate Assembly -- Modular DNA Construct Design for High-Throughput Golden Gate Assembly -- Using ApE for In Silico Golden Gate Cloning -- Standardized Golden Gate Assembly Metadata Representation using SBOL -- Use of a Golden Gate Plasmid Set Enabling Scarless MoClo-Compatible Transcription Unit Assembly -- Biofoundry-Assisted Golden Gate Cloning with AssemblyTron -- Automation and Miniaturization of Golden Gate DNA Assembly Reactions using Acoustic Dispensers -- Validation of Golden Gate Assemblies using Highly Multiplexed Nanopore Amplicon Sequencing -- Modular Golden Gate Assembly of Linear DNA Templates for Cell-Free Prototyping -- Construction of Small Genes with Repetitive Elements using Oligo Extension and Golden Gate Assembly -- Simplifying Recombinant Protein Production: Combining Golden Gate Cloning with a Standardized Protein Purification Scheme -- Generating Site Saturation Mutagenesis Libraries and Transferring Them to Broad Host-Range Plasmids using Golden Gate Cloning -- Combining Oligo Pools and Golden Gate Cloning to Create Protein Variant Libraries or Guide RNA Libraries for CRISPR Applications -- Golden Gate Cloning of Synthetic CRISPR RNA Spacer Sequences -- Golden Gate Cloning of Expression Plasmids for Synthetic Small RNAs in Bacteria -- Utilizing Golden Gate Assembly to Streamline CRISPR-Cas/NgTET-Based Phage Mutagenesis -- Golden Gate Assisted Gene Doctoring for Streamlined and Efficient Recombineering in Bacteria -- Protocol for NT-CRISPR, A Method for Efficient Genome Engineering in Vibrio natriegens -- Golden Gate Cloning in Actinobacteria: Opportunities and Challenges -- Golden Gate Assembly of Transcriptional Unit Libraries into a Rearrangeable Gene Cluster -- Golden Gate Cloning for Efficient Biosynthesis of Lycopene in Synthetic Yeast -- YeastFab Cloning of Toxic Genes and Protein Expression Optimization in Yeast -- Golden Gate Cloning for the Standardized Assembly of Gene Elements with Modular Cloning in Chlamydomonas -- Assembling DNA Plasmids with the Multi-Kingdom (MK) Cloning System -- Advancements in Golden Gate Cloning: A Comprehensive Review |
| Persistent Identifier |
URN: urn:nbn:de:101:1-2410040413157.679944145637 DOI: 10.1007/978-1-0716-4220-7 |
| URL | https://doi.org/10.1007/978-1-0716-4220-7 |
| ISBN/Einband/Preis | 978-1-0716-4220-7 |
| Sprache(n) | Englisch (eng) |
| Beziehungen | Methods in Molecular Biology ; 2850 |
| DDC-Notation | 572.8 (maschinell ermittelte DDC-Kurznotation) |
| Sachgruppe(n) | 570 Biowissenschaften, Biologie |
| Online-Zugriff | Archivobjekt öffnen |
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