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| Link zu diesem Datensatz | https://d-nb.info/1390199959 |
| Titel | Fluorescence In Situ Hybridization (FISH) : Application Guide / edited by Thomas Liehr |
| Person(en) | Liehr, Thomas (Herausgeber) |
| Organisation(en) | SpringerLink (Online service) (Sonstige) |
| Ausgabe | 3rd ed. 2026 |
| Verlag | New York, NY : Springer US, Imprint: Humana |
| Zeitliche Einordnung | Erscheinungsdatum: 2026 |
| Umfang/Format | Online-Ressource, XXI, 727 p. 100 illus. : online resource. |
| Andere Ausgabe(n) |
Printed edition:: ISBN: 978-1-0716-5149-0 Printed edition:: ISBN: 978-1-0716-5151-3 Printed edition:: ISBN: 978-1-0716-5152-0 |
| Inhalt | Background -- Microscopy and imaging: Visualization matters -- Optical filters and light sources for FISH -- Automation of FISH-procedure and evaluation -- Classification of FISH probes -- Commercial FISH probes -- Generation of painting probes from flow-sorted and microdissected chromosomes -- FISH-microdissection -- Homemade locus-specific FISH probes: Bacterial artificial chromosomes -- The standard FISH procedure -- Microwave treatment for better FISH results in a shorter time -- FISH with and without COT DNA -- One-day FISH procedure -- Quantitative FISH (Q-FISH) -- Telomere length measurement by FISH: Image processing techniques -- Directional Genomic Hybridization (dGH) for high-resolution detection of intrachromosomal rearrangements -- The replicative detargeting FISH (ReD-FISH) technique in studies of telomere replication -- Pre- and postnatal diagnostics and research on peripheral blood, bone marrow chorion, amniocytes, and fibroblasts -- Application of FISH to previously GTG-banded and/or embedded cytogenetic slides -- FISH in native amniocytes -- Tumorcytogenetic diagnostics and research on blood and bone marrow smears or effusions -- Characterization of mosaicism in different easy-to-acquire body tissues such as buccal smears, skin abrasions, hair root cells or urine -- Characterization of archived formalin-fixed/paraffin-embedded or cryofixed tissue, including nucleus extraction -- FISH on sperm, spermatocytes and oocytes -- Fluorescence in situ hybridization on DNA fibers: Molecular combing -- Two- to three-color FISH -- Multiplex FISH and spectral karyotyping -- FISH banding techniques -- Centromere directed M-FISH approaches (cenM-FISH) -- Heterochromatin directed M-FISH (HCM-FISH) -- FISH for repeatedly observed euchromatic variants -- Subtelomeric and/or subcentromeric probe sets -- Bar-coding is more actual than ever -- FISH for repeatedly observed constitutional rearrangements -- Parental origin determination FISH: Pod-FISH -- Simultaneous FISH and fluorescence immunostaining for DNA–protein co-detection -- CENP-antibodies used additionally to FISH -- X-inactivation accessed by FISH and EDU-immunostaining -- Immuno-FISH and microspreading technique for synaptonemal complex studies -- Yeast chromosomes revealed by immuno FISH -- Interphase FISH in diagnostics -- Interphase FISH for detection of chromosomal mosaicism and instability -- Comet-FISH -- Micronucleus FISH -- Three-dimensional interphase analysis using suspension FISH -- 3D DNA-FISH protocol for the study of the DUX gene in preimplantation bovine embryos -- Animal probes and Zoo-FISH -- Three-dimensional co-immuno and RNA-fluorescence in situ hybridization in preimplantation mouse embryos -- FISH in bird chromosomes: An updated protocol and best practices -- An updated standard protocol for FISH mapping in fish species (fish-FISH) -- FISH in lampbrush-chromosomes -- General protocol for FISH on insect chromosomes -- FISH in Drosophila -- FISH on insect cells transfected with heterologous DNA – a single cell directed approach -- Fluorescence in situ hybridization for plants: Methods of chromosomal preparation -- Mitochondrial DNA (MtDNA) detection by FISH -- Advances in the application of FISH in food microbiology -- Evaluation of polymicrobial involvement using Fluorescence In Situ Hybridization (FISH) in clinical practice -- Comparative genomic hybridization (CGH) and microdissection-based CGH (micro-CGH) in human -- Comparative genomic hybridization (CGH) in animals -- Breakpoint mapping of balanced chromosomal rearrangements using array-CGH with microdissection-derived FISH probes -- Array CGH -- Chromosome microarray results to be verified by FISH -- Sequencing of microdissection derived FISH-probes -- Next generation sequencing should consider molecular cytogenetic results -- Optical genome mapping results need to be verified by FISH -- Application of ALFA-tag signal amplification to expand the CRISPR-based DNA imaging toolkit |
| Persistent Identifier |
URN: urn:nbn:de:101:1-2602140309431.866538169191 DOI: 10.1007/978-1-0716-5150-6 |
| URL | https://doi.org/10.1007/978-1-0716-5150-6 |
| ISBN/Einband/Preis | 978-1-0716-5150-6 |
| Sprache(n) | Englisch (eng) |
| Beziehungen | Springer Protocols Handbooks |
| Schlagwörter | Cytogenetik ; Molekulargenetik ; Fluoreszenz-in-situ-Hybridisierung |
| DDC-Notation | 572.8 [DDC22ger] |
| Sachgruppe(n) | 570 Biowissenschaften, Biologie |
| Online-Zugriff | Archivobjekt öffnen |
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