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| Link zu diesem Datensatz | https://d-nb.info/1371224374 |
| Titel | Biophysical Chemistry of Proteins : An Introduction to Laboratory Methods / by Engelbert Buxbaum |
| Person(en) | Buxbaum, Engelbert (Verfasser) |
| Organisation(en) | SpringerLink (Online service) (Sonstige) |
| Ausgabe | 2nd ed. 2025 |
| Verlag | New York, NY : Springer US, Imprint: Springer |
| Zeitliche Einordnung | Erscheinungsdatum: 2025 |
| Umfang/Format | Online-Ressource, VIII, 437 p. 355 illus., 313 illus. in color. : online resource. |
| Andere Ausgabe(n) |
Printed edition:: ISBN: 978-1-0716-4123-1 Printed edition:: ISBN: 978-1-0716-4125-5 Printed edition:: ISBN: 978-1-0716-4126-2 |
| Inhalt | Analytical techniques -- Microscopy -- Single molecule techniques -- Preparation of cells and tissues for microscopy -- Principles of optical spectroscopy -- Photometry -- Fluorimetry -- Chemiluminescence -- Electrophoresis -- Immunological methods -- Isotope techniques -- Purification of proteins -- Homogenisation and fractionisation of cells and tissues -- Isolation of organelles -- Precipitation methods -- Chromatography -- Membrane proteins -- Determination of protein concentration -- Cell culture -- Protein modification and inactivation -- General technical remarks -- Amine-reactive reagents -- Thiol- and disulphide reactive reagents -- Reagents for other groups -- Cross-linkers -- Detection methods -- Spontaneous reactions in proteins -- Protein size and shape -- Centrifugation -- Osmotic pressure -- Diffusion -- Viscosity -- Non-resonant interactions with electromagnetic waves -- Protein structure -- Protein sequencing -- Synthesis of peptides -- Protein secondary structure -- Structure of protein-ligand complexes -- 3D-structures -- Folding and unfolding of proteins -- Enzyme kinetics -- Steady-state kinetics -- Leaving the steady state: Analysis of progress curves -- Reaction velocities -- Isotope effects -- Isotope exchange -- Protein-ligand interactions -- General conditions for interpretable results -- Binding equations -- Methods to measure binding equilibria -- Temperature effects on binding equilibrium and reaction rate -- Industrial enzymology -- Industrial enzyme use -- Immobilised enzymes -- Special statistics -- Quality control -- Testing whether or not a model fits the data -- Appendix -- List of symbols -- Greek alphabet -- Properties of electrophoretic buffers -- Bond properties -- Acronyms |
| Persistent Identifier |
URN: urn:nbn:de:101:1-2507110407267.316901779165 DOI: 10.1007/978-1-0716-4124-8 |
| URL | https://doi.org/10.1007/978-1-0716-4124-8 |
| ISBN/Einband/Preis | 978-1-0716-4124-8 |
| Sprache(n) | Englisch (eng) |
| DDC-Notation | 535.4 (maschinell ermittelte DDC-Kurznotation) |
| Sachgruppe(n) | 530 Physik |
| Online-Zugriff | Archivobjekt öffnen |
Treffer 61 von 37982
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